解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12888
更新日期:2018-08-01 00:00:00
abstract::Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12880
更新日期:2018-08-01 00:00:00
abstract::Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12875
更新日期:2018-07-01 00:00:00
abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12878
更新日期:2018-07-01 00:00:00
abstract::Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12855
更新日期:2018-06-01 00:00:00
abstract::Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed stor...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12851
更新日期:2018-05-01 00:00:00
abstract::Cellulose is an abundant biopolymer and a prominent constituent of plant cell walls. Cellulose is also a central component to plant morphogenesis and contributes the bulk of a plant's biomass. While cellulose synthase (CesA) genes were identified over two decades ago, genetic manipulation of this family to enhance cel...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12842
更新日期:2018-05-01 00:00:00
abstract::Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12839
更新日期:2018-04-01 00:00:00
abstract::To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12896
更新日期:2018-03-05 00:00:00
abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12901
更新日期:2018-02-24 00:00:00
abstract::Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting r...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12899
更新日期:2018-02-12 00:00:00
abstract::Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12787
更新日期:2018-02-01 00:00:00
abstract::Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12795
更新日期:2018-02-01 00:00:00
abstract::Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting str...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12774
更新日期:2018-01-01 00:00:00
abstract::Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistanc...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12733
更新日期:2017-12-01 00:00:00
abstract::GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12798
更新日期:2017-10-01 00:00:00
abstract::The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12714
更新日期:2017-10-01 00:00:00
abstract::Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt crops in agriculture. The fitness of Bt-resistant insects on Bt and non-Bt plants is a key parameter that determines the development of Bt resistance in insect ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12718
更新日期:2017-10-01 00:00:00
abstract::There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12703
更新日期:2017-09-01 00:00:00
abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12693
更新日期:2017-08-01 00:00:00
abstract::Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,meta分析
doi:10.1111/pbi.12698
更新日期:2017-08-01 00:00:00
abstract::The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12684
更新日期:2017-07-01 00:00:00
abstract::The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12674
更新日期:2017-06-01 00:00:00
abstract::Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12658
更新日期:2017-05-01 00:00:00
abstract::Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial rele...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12660
更新日期:2017-05-01 00:00:00
abstract::The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12610
更新日期:2017-02-01 00:00:00
abstract::Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12581
更新日期:2016-12-01 00:00:00
abstract::Next-generation sequencing of complete genomes has given researchers unprecedented levels of information to study the multifaceted evolutionary changes that have shaped elite plant germplasm. In conjunction with population genetic analytical techniques and detailed online databases, we can more accurately capture the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12578
更新日期:2016-12-01 00:00:00
abstract::Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12510
更新日期:2016-07-01 00:00:00
abstract::The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12513
更新日期:2016-07-01 00:00:00
abstract::Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12515
更新日期:2016-07-01 00:00:00
abstract::Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12503
更新日期:2016-06-01 00:00:00
abstract::Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the ant...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12507
更新日期:2016-06-01 00:00:00
abstract::Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12506
更新日期:2016-06-01 00:00:00
abstract::In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12488
更新日期:2016-05-01 00:00:00
abstract::Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very success...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12466
更新日期:2016-05-01 00:00:00
abstract::Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12493
更新日期:2016-05-01 00:00:00
abstract::Woody biomass has gained popularity as an environmentally friendly, renewable and sustainable resource for liquid fuel production. Here, we demonstrate biotechnological improvement of the quantity and quality of woody biomass by employing developing xylem (DX)-preferential production of gibberellin (GA), a phytohormon...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12484
更新日期:2016-04-01 00:00:00
abstract::Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12483
更新日期:2016-04-01 00:00:00
abstract::As an increasing number of genome sequences become available for a wide range of species, there is a growing understanding that the genome of a single individual is insufficient to represent the gene diversity within a whole species. Many studies examine the sequence diversity within genes, and this allelic variation ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12499
更新日期:2016-04-01 00:00:00